Real-Time Nanoplasmonic Sensing of Three-Dimensional Morphological Changes in a Supported Lipid Bilayer and Antimicrobial Testing Applications Full article
| Journal |
Biosensors and Bioelectronics
ISSN: 0956-5663 , E-ISSN: 1873-4235 |
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| Output data | Year: 2021, Volume: 174, Article number : 112768, Pages count : 8 DOI: 10.1016/j.bios.2020.112768 | ||||||
| Tags | nanoplasmonics; localized surface plasmon resonance; label-free biosensing; artificial cell membrane; antimicrobia | ||||||
| Authors |
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| Affiliations |
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Funding (2)
| 1 | National Research Foundation of Korea | 2020R1C1C1004385 |
| 2 | National Research Foundation of Korea | 2019H1D3A1A01070318 |
Abstract:
We report the development of a real-time localized surface plasmon resonance (LSPR) biosensing strategy to detect three-dimensional morphological changes in a supported lipid bilayer (SLB) on a plasmonic substrate. The sensing concept advances on past efforts to detect subtle conformational changes in adsorbed biomacromolecules by demonstrating the capability to track large-scale, complex adsorbate shape changes and to classify different types of shape changes based on specific, multi-step measurement signatures. To validate this concept, we tested the addition of antimicrobial fatty acids, monoglycerides, and surfactants in micellar form to the SLB platform, which triggered specific three-dimensional membrane morphological changes such as tubule or bud formation along with solubilization. Experimentally, the different remodeling events were detected by distinct measurement signatures related to the shape and size of lipid protrusions that formed and evolved over time, which agreed well with a newly developed theoretical model. Our conceptual approach and formalism are applicable to various biosensing techniques, including not only LSPR but also surface plasmon resonance (SPR) and total internal reflection fluorescence (TIRF) microscopy. These sensing capabilities are advantageous for evaluating the mechanisms of antimicrobial drug candidates and other membrane-active compounds, and the measurement strategy is extendable to a wide range of biomimetic lipid compositions.
Cite:
Yoon B.K.
, Park H.
, Zhdanov V.P.
, Jackman J.A.
, Cho N-J.
Real-Time Nanoplasmonic Sensing of Three-Dimensional Morphological Changes in a Supported Lipid Bilayer and Antimicrobial Testing Applications
Biosensors and Bioelectronics. 2021. V.174. 112768 :1-8. DOI: 10.1016/j.bios.2020.112768 WOS Scopus РИНЦ AN PMID OpenAlex
Real-Time Nanoplasmonic Sensing of Three-Dimensional Morphological Changes in a Supported Lipid Bilayer and Antimicrobial Testing Applications
Biosensors and Bioelectronics. 2021. V.174. 112768 :1-8. DOI: 10.1016/j.bios.2020.112768 WOS Scopus РИНЦ AN PMID OpenAlex
Dates:
| Submitted: | Aug 6, 2020 |
| Accepted: | Oct 23, 2020 |
| Published online: | Oct 31, 2020 |
| Published print: | Feb 15, 2021 |
Identifiers:
| Web of science: | WOS:000605488400009 |
| Scopus: | 2-s2.0-85097229358 |
| Elibrary: | 45100230 |
| Chemical Abstracts: | 2020:2517680 |
| PMID: | 33288427 |
| OpenAlex: | W3097403934 |