Study on Physicochemical Properties of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglycerides’ Interesterification Full article
| Journal |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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| Output data | Year: 2014, Volume: 50, Number: 7, Pages: 709-721 Pages count : 13 DOI: 10.1134/S0003683814070047 | ||||||
| Tags | cell lysates, entrapment inside silica xerogel, recombinant thermostable lipase, triglycerides’ interesterification, whole-cell biocatalysts | ||||||
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Abstract:
The physicochemical properties of multicomponent biocatalysts for triglycerides’ interesterification have been studied. They were prepared by entrapment of partially or completely destructed cells of a recombinant rE.coli/lip strain—the producer of a thermostable lipase from Thermomyces lanuginosus⎯inside silica xerogel. The functional role and optimum contents of components included such as whole cells or cells’ lysates, water, water-retaining agents, and nanostructured carbon forms (nanotubes, nanospheres) were investigated. The optimum composition of biocatalysts prepared on the basis of rE.coli/lip cells’ lysates, which possess enzymatic activity in water-free media, was selected. The half-inactivation time of the prepared biocatalysts in the process of interesterification of oil-fat blends in a flow packed-bed reactor was ~70 h at 70–75°C.
Cite:
Kovalenko G.A.
, Perminova L.V.
, Beklemishev A.B.
, Tkachenko V.I.
Study on Physicochemical Properties of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglycerides’ Interesterification
Applied Biochemistry and Microbiology. 2014. V.50. N7. P.709-721. DOI: 10.1134/S0003683814070047 WOS Scopus РИНЦ ANCAN OpenAlex
Study on Physicochemical Properties of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglycerides’ Interesterification
Applied Biochemistry and Microbiology. 2014. V.50. N7. P.709-721. DOI: 10.1134/S0003683814070047 WOS Scopus РИНЦ ANCAN OpenAlex
Original:
Коваленко Г.А.
, Перминова Л.В.
, Беклемишев А.Б.
, Ткаченко В.И.
Исследование физико-химических свойств биокатализаторов с активностью термостабильной липазы и конечных продуктов переэтерификации триглицеридов
Биотехнология. 2013. №6. С.35-50. RSCI РИНЦ AN
Исследование физико-химических свойств биокатализаторов с активностью термостабильной липазы и конечных продуктов переэтерификации триглицеридов
Биотехнология. 2013. №6. С.35-50. RSCI РИНЦ AN
Dates:
| Submitted: | Oct 25, 2013 |
| Published online: | Oct 21, 2014 |
| Published print: | Dec 1, 2014 |
Identifiers:
| Web of science: | WOS:000344160100003 |
| Scopus: | 2-s2.0-84920948197 |
| Elibrary: | 24029914 |
| Chemical Abstracts: | 2014:1793366 |
| Chemical Abstracts (print): | 162:522377 |
| OpenAlex: | W2073620844 |