Study on Physicochemical Properties of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglycerides’ Interesterification Научная публикация
Журнал |
Applied Biochemistry and Microbiology
ISSN: 0003-6838 , E-ISSN: 1608-3024 |
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Вых. Данные | Год: 2014, Том: 50, Номер: 7, Страницы: 709-721 Страниц : 13 DOI: 10.1134/S0003683814070047 | ||||||
Ключевые слова | cell lysates, entrapment inside silica xerogel, recombinant thermostable lipase, triglycerides’ interesterification, whole-cell biocatalysts | ||||||
Авторы |
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Организации |
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Реферат:
The physicochemical properties of multicomponent biocatalysts for triglycerides’ interesterification have been studied. They were prepared by entrapment of partially or completely destructed cells of a recombinant rE.coli/lip strain—the producer of a thermostable lipase from Thermomyces lanuginosus⎯inside silica xerogel. The functional role and optimum contents of components included such as whole cells or cells’ lysates, water, water-retaining agents, and nanostructured carbon forms (nanotubes, nanospheres) were investigated. The optimum composition of biocatalysts prepared on the basis of rE.coli/lip cells’ lysates, which possess enzymatic activity in water-free media, was selected. The half-inactivation time of the prepared biocatalysts in the process of interesterification of oil-fat blends in a flow packed-bed reactor was ~70 h at 70–75°C.
Библиографическая ссылка:
Kovalenko G.A.
, Perminova L.V.
, Beklemishev A.B.
, Tkachenko V.I.
Study on Physicochemical Properties of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglycerides’ Interesterification
Applied Biochemistry and Microbiology. 2014. V.50. N7. P.709-721. DOI: 10.1134/S0003683814070047 WOS Scopus РИНЦ CAPlusCA OpenAlex
Study on Physicochemical Properties of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglycerides’ Interesterification
Applied Biochemistry and Microbiology. 2014. V.50. N7. P.709-721. DOI: 10.1134/S0003683814070047 WOS Scopus РИНЦ CAPlusCA OpenAlex
Оригинальная:
Коваленко Г.А.
, Перминова Л.В.
, Беклемишев А.Б.
, Ткаченко В.И.
Исследование физико-химических свойств биокатализаторов с активностью термостабильной липазы и конечных продуктов переэтерификации триглицеридов
Биотехнология. 2013. №6. С.35-50. RSCI РИНЦ CAPlus
Исследование физико-химических свойств биокатализаторов с активностью термостабильной липазы и конечных продуктов переэтерификации триглицеридов
Биотехнология. 2013. №6. С.35-50. RSCI РИНЦ CAPlus
Даты:
Поступила в редакцию: | 25 окт. 2013 г. |
Опубликована online: | 21 окт. 2014 г. |
Опубликована в печати: | 1 дек. 2014 г. |
Идентификаторы БД:
Web of science: | WOS:000344160100003 |
Scopus: | 2-s2.0-84920948197 |
РИНЦ: | 24029914 |
Chemical Abstracts: | 2014:1793366 |
Chemical Abstracts (print): | 162:522377 |
OpenAlex: | W2073620844 |