Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles | Sciact - CRIS-система Института катализа

Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles Письма в редакцию

Журнал

Nano Letters
ISSN: 1530-6984 , E-ISSN: 1530-6992

Вых. Данные

Год: 2021, Том: 21, Номер: 11, Страницы: 4622–4628 Страниц : 7 DOI: 10.1021/acs.nanolett.1c00644

Ключевые слова

surface-sensitive scattering microscopy; surface plasmon resonance; single nanoparticle analytics; protein adsorption kinetics

Авторы

Sjöberg Mattias ¹ , Mapar Mokhtar ¹ , Armanious Antonius ¹ , Zhdanov Vladimir P. ^1,2 , Agnarsson Björn ¹ , Höök Fredrik ¹

Организации

1	Division of Nano and Biophysics, Department of Physics, Chalmers University of Technology, Gothenburg 41296, Sweden
2	Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia

Информация о финансировании (3)

1	Stiftelsen för strategisk forskning	IRC15-0065
2	Swedish Research Council	2018-04900
3	Фонд Кнута и Элис Валленберг	2019-0577

In-depth understanding of the intricate interactions between biomolecules and nanoparticles is hampered by a lack of analytical methods providing quantitative information about binding kinetics. Herein, we demonstrate how label-free evanescent light-scattering microscopy can be used to temporally resolve specific protein binding to individual surface-bound (∼100 nm) lipid vesicles. A theoretical model is proposed that translates protein-induced changes in light-scattering intensity into bound mass. Since the analysis is centered on individual lipid vesicles, the signal from nonspecific protein binding to the surrounding surface is completely avoided, offering a key advantage over conventional surface-based techniques. Further, by averaging the intensities from less than 2000 lipid vesicles, the sensitivity is shown to increase by orders of magnitude. Taken together, these features provide a new avenue in studies of protein-nanoparticle interaction, in general, and specifically in the context of nanoparticles in medical diagnostics and drug delivery.

Sjöberg M. , Mapar M. , Armanious A. , Zhdanov V.P. , Agnarsson B. , Höök F.
Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles

Nano Letters. 2021. V.21. N11. P.4622–4628. DOI: 10.1021/acs.nanolett.1c00644 WOS Scopus РИНЦ CAPlus PMID OpenAlex

Полный текст от издателя

Поступила в редакцию:	15 февр. 2021 г.
Принята к публикации:	5 мая 2021 г.
Опубликована online:	18 мая 2021 г.
Опубликована в печати:	9 июн. 2021 г.

Web of science:	WOS:000662078500015
Scopus:	2-s2.0-85108020955
РИНЦ:	46830211
Chemical Abstracts:	2021:1100148
PMID (PubMed):	34003003
OpenAlex:	W3162913651

БД	Цитирований
Scopus	8
Web of science	8
РИНЦ	7
OpenAlex	12