Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles ArticleGenre_short.LETTER
| Journal |
Nano Letters
ISSN: 1530-6984 , E-ISSN: 1530-6992 |
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| Output data | Year: 2021, Volume: 21, Number: 11, Pages: 4622–4628 Pages count : 7 DOI: 10.1021/acs.nanolett.1c00644 | ||||
| Tags | surface-sensitive scattering microscopy; surface plasmon resonance; single nanoparticle analytics; protein adsorption kinetics | ||||
| Authors |
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| Affiliations |
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Funding (3)
| 1 | Stiftelsen för strategisk forskning | IRC15-0065 |
| 2 | Swedish Research Council | 2018-04900 |
| 3 | Knut and Alice Wallenberg Foundation | 2019-0577 |
Abstract:
In-depth understanding of the intricate interactions between biomolecules and nanoparticles is hampered by a lack of analytical methods providing quantitative information about binding kinetics. Herein, we demonstrate how label-free evanescent light-scattering microscopy can be used to temporally resolve specific protein binding to individual surface-bound (∼100 nm) lipid vesicles. A theoretical model is proposed that translates protein-induced changes in light-scattering intensity into bound mass. Since the analysis is centered on individual lipid vesicles, the signal from nonspecific protein binding to the surrounding surface is completely avoided, offering a key advantage over conventional surface-based techniques. Further, by averaging the intensities from less than 2000 lipid vesicles, the sensitivity is shown to increase by orders of magnitude. Taken together, these features provide a new avenue in studies of protein-nanoparticle interaction, in general, and specifically in the context of nanoparticles in medical diagnostics and drug delivery.
Cite:
Sjöberg M.
, Mapar M.
, Armanious A.
, Zhdanov V.P.
, Agnarsson B.
, Höök F.
Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles
Nano Letters. 2021.
V.21. N11. P.4622–4628. DOI: 10.1021/acs.nanolett.1c00644
WOS
Scopus
РИНЦ
AN
PMID
OpenAlex
Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles
Nano Letters. 2021.
V.21. N11. P.4622–4628. DOI: 10.1021/acs.nanolett.1c00644
WOS
Scopus
РИНЦ
AN
PMID
OpenAlex
Files:
Full text from publisher
Dates:
| Submitted: | Feb 15, 2021 |
| Accepted: | May 5, 2021 |
| Published online: | May 18, 2021 |
| Published print: | Jun 9, 2021 |
Identifiers:
| Web of science: | WOS:000662078500015 |
| Scopus: | 2-s2.0-85108020955 |
| Elibrary: | 46830211 |
| Chemical Abstracts: | 2021:1100148 |
| PMID: | 34003003 |
| OpenAlex: | W3162913651 |