Label-Free Quantification of Protein Binding to Lipid Vesicles Using Transparent Waveguide Evanescent-Field Scattering Microscopy with Liquid Control Научная публикация
| Журнал |
Biomedical Optics Express
ISSN: 2156-7085 |
||||||
|---|---|---|---|---|---|---|---|
| Вых. Данные | Год: 2023, Том: 14, Номер: 8, Номер статьи : 4003, Страниц : 14 DOI: 10.1364/boe.490051 | ||||||
| Ключевые слова | Biochemistry; Controlled drug delivery;Fluorescence;Light scattering;Nanoparticles;Proteins;Targeted drug delivery; Viruses; Waveguides | ||||||
| Авторы |
|
||||||
| Организации |
|
Информация о финансировании (3)
| 1 | Фонд Кнута и Элис Валленберг | 2019-0577 |
| 2 | Swedish Research Council | 2018-04900 |
| 3 | Chalmers University of Technology |
Реферат:
Recent innovations in microscopy techniques are paving the way for label-free studies of single nanoscopic biological entities such as viruses, lipid-nanoparticle drug carriers, and even proteins. One such technique is waveguide evanescent-field microscopy, which offers a relatively simple, yet sensitive, way of achieving label-free light scattering-based imaging of nanoparticles on surfaces. Herein, we extend the application of this technique by incorporating microfluidic liquid control and adapting the design for use with inverted microscopes by fabricating a waveguide on a transparent substrate. We furthermore formulate analytical models describing scattering and fluorescence intensities from single spherical and shell-like objects interacting with evanescent fields. The models are then applied to analyze scattering and fluorescence intensities from adsorbed polystyrene beads and to temporally resolve cholera-toxin B (CTB) binding to individual surface-immobilized glycosphingolipid GM1 containing vesicles. We also propose a self-consistent means to quantify the thickness of the CTB layer, revealing that protein-binding to individual vesicles can be characterized with sub-nm precision in a time-resolved manner.
Библиографическая ссылка:
Mapar M.
, Sjöberg M.
, Zhdanov V.P.
, Agnarsson B.
, Höök F.
Label-Free Quantification of Protein Binding to Lipid Vesicles Using Transparent Waveguide Evanescent-Field Scattering Microscopy with Liquid Control
Biomedical Optics Express. 2023. V.14. N8. 4003 :1-14. DOI: 10.1364/boe.490051 WOS Scopus РИНЦ CAPlus PMID OpenAlex
Label-Free Quantification of Protein Binding to Lipid Vesicles Using Transparent Waveguide Evanescent-Field Scattering Microscopy with Liquid Control
Biomedical Optics Express. 2023. V.14. N8. 4003 :1-14. DOI: 10.1364/boe.490051 WOS Scopus РИНЦ CAPlus PMID OpenAlex
Даты:
| Поступила в редакцию: | 4 апр. 2023 г. |
| Принята к публикации: | 18 июн. 2023 г. |
| Опубликована online: | 10 июл. 2023 г. |
| Опубликована в печати: | 1 авг. 2023 г. |
Идентификаторы БД:
| Web of science: | WOS:001052308700001 |
| Scopus: | 2-s2.0-85168992246 |
| РИНЦ: | 54368753 |
| Chemical Abstracts: | 2023:2104834 |
| PMID (PubMed): | 37799672 |
| OpenAlex: | W4381800447 |