Label-Free Quantification of Protein Binding to Lipid Vesicles Using Transparent Waveguide Evanescent-Field Scattering Microscopy with Liquid Control Full article
| Journal |
Biomedical Optics Express
ISSN: 2156-7085 |
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| Output data | Year: 2023, Volume: 14, Number: 8, Article number : 4003, Pages count : 14 DOI: 10.1364/boe.490051 | ||||||
| Tags | Biochemistry; Controlled drug delivery;Fluorescence;Light scattering;Nanoparticles;Proteins;Targeted drug delivery; Viruses; Waveguides | ||||||
| Authors |
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| Affiliations |
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Funding (3)
| 1 | Knut and Alice Wallenberg Foundation | 2019-0577 |
| 2 | Swedish Research Council | 2018-04900 |
| 3 | Chalmers University of Technology |
Abstract:
Recent innovations in microscopy techniques are paving the way for label-free studies of single nanoscopic biological entities such as viruses, lipid-nanoparticle drug carriers, and even proteins. One such technique is waveguide evanescent-field microscopy, which offers a relatively simple, yet sensitive, way of achieving label-free light scattering-based imaging of nanoparticles on surfaces. Herein, we extend the application of this technique by incorporating microfluidic liquid control and adapting the design for use with inverted microscopes by fabricating a waveguide on a transparent substrate. We furthermore formulate analytical models describing scattering and fluorescence intensities from single spherical and shell-like objects interacting with evanescent fields. The models are then applied to analyze scattering and fluorescence intensities from adsorbed polystyrene beads and to temporally resolve cholera-toxin B (CTB) binding to individual surface-immobilized glycosphingolipid GM1 containing vesicles. We also propose a self-consistent means to quantify the thickness of the CTB layer, revealing that protein-binding to individual vesicles can be characterized with sub-nm precision in a time-resolved manner.
Cite:
Mapar M.
, Sjöberg M.
, Zhdanov V.P.
, Agnarsson B.
, Höök F.
Label-Free Quantification of Protein Binding to Lipid Vesicles Using Transparent Waveguide Evanescent-Field Scattering Microscopy with Liquid Control
Biomedical Optics Express. 2023. V.14. N8. 4003 :1-14. DOI: 10.1364/boe.490051 WOS Scopus РИНЦ AN PMID OpenAlex
Label-Free Quantification of Protein Binding to Lipid Vesicles Using Transparent Waveguide Evanescent-Field Scattering Microscopy with Liquid Control
Biomedical Optics Express. 2023. V.14. N8. 4003 :1-14. DOI: 10.1364/boe.490051 WOS Scopus РИНЦ AN PMID OpenAlex
Dates:
| Submitted: | Apr 4, 2023 |
| Accepted: | Jun 18, 2023 |
| Published online: | Jul 10, 2023 |
| Published print: | Aug 1, 2023 |
Identifiers:
| Web of science: | WOS:001052308700001 |
| Scopus: | 2-s2.0-85168992246 |
| Elibrary: | 54368753 |
| Chemical Abstracts: | 2023:2104834 |
| PMID: | 37799672 |
| OpenAlex: | W4381800447 |