Evaluation of Properties for Carbothane™ 3575A–Based Electrospun Vascular Grafts in vitro and in vivo | Sciact - CRIS-system of Boreskov Institute of Catalysis

Evaluation of Properties for Carbothane™ 3575A–Based Electrospun Vascular Grafts in vitro and in vivo Full article

Journal

Biomedical Materials
ISSN: 1748-605X

Output data

Year: 2024, Volume: 19, Number: 6, Article number : 065012, Pages count : DOI: 10.1088/1748-605x/ad792d

Tags

Carbothane™ 3575A; electrospinning; endothelial human cells; functioning in vivo; hemocompatibility; polycarbonate-based polyurethane; small-diameter vascular graft.

Authors

Chernonosova Vera S. ¹ , Osipova Olesia S. ² , Nuankai Zhou ¹ , Shundrina Inna K. ³ , Murashov Ivan S. ² , Larichev Yurii V. ⁴ , Karpenko Andrey A. ² , Laktionov Pavel P. ¹

Affiliations

1	Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Russian Academy of Sciences, 8 Lavrentiev Avenue, Novosibirsk, Novosibirskaâ, 630090, RUSSIAN FEDERATION.
2	FSBI National Medical Research Center named after E N Meshalkin, Rechkunovskaya 15, Novosibirsk, None Selected, 630055, RUSSIAN FEDERATION.
3	Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentieva Avenue 9, Novosibirsk, 630090, RUSSIAN FEDERATION.
4	Boreskov Institute of Catalysis SB RAS, Lavrentieva Avenue 5, Novosibirsk, 630090, RUSSIAN FEDERATION.

Bioengineered vascular grafts have emerged as a promising alternative to the treatment of damaged or occlusive vessels. It is thought that polyurethane-based scaffolds possess suitable hemocompatibility and biomechanics comparable to those of normal blood vessels. In this study, we investigated the properties of electrospun scaffolds comprising various blends of biostable polycarbonate-based polyurethane (Carbothane™ 3575A) and gelatin. Scaffolds were characterized by scanning electron microscopy, infra-red spectroscopy, small-angle X-ray scattering, stress-loading tests, and interactions with primary human cells and blood. Data from in vitro experiments demonstrated that a scaffold produced from a blend of 5% Carbothane™ 3575A and 10% gelatin has proven to be a suitable material for fabricating a small-diameter vascular graft. A comparative in vivo study of such vascular grafts and ePTFE grafts implanted in the abdominal aorta of Wistar rats was performed. The data of intravital study and histological examination indicated that Carbothane-based electrospun grafts outclass ePTFE grafts and represent a promising device for preclinical studies to satisfy vascular surgery needs.

Chernonosova V.S. , Osipova O.S. , Nuankai Z. , Shundrina I.K. , Murashov I.S. , Larichev Y.V. , Karpenko A.A. , Laktionov P.P.
Evaluation of Properties for Carbothane™ 3575A–Based Electrospun Vascular Grafts in vitro and in vivo
Biomedical Materials. 2024. V.19. N6. 065012 . DOI: 10.1088/1748-605x/ad792d WOS Scopus РИНЦ PMID OpenAlex

Submitted:	Apr 13, 2024
Accepted:	Sep 10, 2024
Published online:	Sep 10, 2024
Published print:	Nov 1, 2024

Web of science:	WOS:001317243000001
Scopus:	2-s2.0-85204660041
Elibrary:	73859719
PMID:	39255825
OpenAlex:	W4402397827

DB	Citing
OpenAlex	1
Web of science	1
Scopus	1