Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids Научная публикация
Журнал |
Biophysics
ISSN: 0006-3509 , E-ISSN: 1555-6654 |
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Вых. Данные | Год: 2000, Том: 45, Номер: 4, Страницы: 594-602 Страниц : 9 | ||||
Ключевые слова | DNA, APOLIPOPROTEIN A-I, (APOLIPOPROTEIN A-I)-GLUCOCORTICOID COMPLEXES, INTERACTION | ||||
Авторы |
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Организации |
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Реферат:
A biochemically active complex of apobpoprotein A-I with tetrahydrocortisol was revealed and found to stimulate gene expression in hepatocytes. In native DNA isolated from rat liver, single-stranded regions appeared upon titration with a complex (apolipoprotein A-I)-tetrahydrocortisol, as demonstrated with a fluorescent probe. The small-angle X-ray scattering confirmed that the complex had an effect on the DNA secondary structure. With tetrahydrocortisol serving as a ligand, about 54 apolipoprotein A-I molecules were bound to a DNA molecule to break the hydrogen bonds between the heterocyclic base pairs. We suggest that a new biochemical mechanism underlies the cooperative effect of the high-density lipoproteins and Cortisol in the regulation of gene expression in hepatocytes, which also involves resident liver macrophages. This mechanism implies DNA interaction with the (apolipoprotein A-I)-tetrahydrocortisol complex, appearance of single-stranded DNA regions in the binding sites, and subsequent initiation of gene transcription.
Библиографическая ссылка:
Panin L.E.
, Tuzikov F.V.
, Tuzikova N.A.
, Gimautdinova O.I.
, Polyakov L.M.
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Biophysics. 2000. V.45. N4. P.594-602. РИНЦ
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Biophysics. 2000. V.45. N4. P.594-602. РИНЦ
Оригинальная:
Panin L.E.
, Tuzikov F.V.
, Tuzikova N.A.
, Gimautdinova O.I.
, Polyakov L.M.
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Биофизика. 2000. V.45. N4. P.618-619. РИНЦ
Interaction of Eukaryotic DNA with Apolipoprotein A-I and Its Complexes with Glucocorticoids
Биофизика. 2000. V.45. N4. P.618-619. РИНЦ
Идентификаторы БД:
РИНЦ | 22589361 |
Цитирование в БД:
БД | Цитирований |
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РИНЦ | 1 |